Exploring the feasibility of a single-protoplast proteomic analysis

Exploring the feasibility of a single-protoplast proteomic analysis

Blog Article

Abstract Background Recent advances in high-resolution mass spectrometry have now enabled the study of proteomes at the single-cell level, offering the potential to unveil novel aspects of cellular processes.Remarkably, there has been no prior attempt to investigate single-plant cell proteomes.In this study, we aimed to explore the feasibility of conducting a proteomic analysis on individual protoplasts.

Findings As a result, our analysis identified 978 proteins from the 180 protoplasts, aligning with well-known biological processes in plant leaves, such as photosynthetic electron transport in photosystem II.Employing the SCP package whelen arges spotlight in the SCoPE2 workflow revealed a notable batch effect and extensive missing values in the data.Following correction, we observed the heterogeneity in single-protoplast proteome expression.

Comparing the results of single-protoplast proteomics with those of bulk leaf proteomics, we noted that only a small fraction of bulk data was detected in the single-protoplast proteomics data, highlighting a technical limitation of the current single-cell proteomics method.Conclusions In summary, we demonstrated the feasibility of conducting a single-protoplast proteomic experiment, revealing heterogeneity in plant cellular proteome expression.This underscores the importance of analyzing a substantial number of plant cells to discern statistically significant changes in plant cell proteomes upon perturbation lovesense 3 such as abscisic acid treatment in future studies.

We anticipate that our study will contribute to advancing single-protoplast proteomics in the near future.